睫状神经营养因子和神经生长因子对人神经母细胞瘤细胞神经元表型的协同和相互作用效应。

Collaborative and reciprocal effects of ciliary neurotrophic factor and nerve growth factor on the neuronal phenotype of human neuroblastoma cells.

作者信息

Zinman L H, Lawrance G, Wang W, Verge V M, Dow K E, Maurice D H, Richardson P M, Riopelle R J

机构信息

Department of Medicine, Queen's University, Kingston, Ontario, Canada.

出版信息

J Neurochem. 1998 Apr;70(4):1411-20. doi: 10.1046/j.1471-4159.1998.70041411.x.

DOI:10.1046/j.1471-4159.1998.70041411.x

PMID:9523557

Abstract

We have probed the molecular basis of functional effects of ciliary neurotrophic factor (CNTF) and nerve growth factor (NGF) on aspects of the neuronal differentiation of LA-N-2 neuroblastoma cells. The influence of CNTF on the cholinergic phenotype can be accounted for by transcriptional/translational effects without implicating posttranslational mechanisms. Although both NGF receptors are expressed constitutively by LA-N-2 cells, CNTF has a marked stimulatory effect on trkA mRNA and protein. The NGF receptors are functional in serum-free conditions where they mitigate CNTF effects on cell adhesion but do not support process extension. Following priming by CNTF, NGF and CNTF have synergistic influences on process formation but not on choline acetyltransferase-specific activity.

摘要

我们探究了睫状神经营养因子（CNTF）和神经生长因子（NGF）对LA-N-2神经母细胞瘤细胞神经元分化各方面功能影响的分子基础。CNTF对胆碱能表型的影响可通过转录/翻译效应来解释，而无需涉及翻译后机制。尽管LA-N-2细胞组成性表达两种NGF受体，但CNTF对trkA mRNA和蛋白有显著的刺激作用。NGF受体在无血清条件下起作用，在此条件下它们可减轻CNTF对细胞黏附的影响，但不支持突起延伸。经CNTF预处理后，NGF和CNTF对突起形成有协同影响，但对胆碱乙酰转移酶特异性活性没有协同影响。