Wilanowski T M, Gibson J B
Molecular Genetics and Evolution Group, Research School of Biological Sciences, The Australian National University, G.P.O. Box 475, Canberra, ACT 2601, Australia.
Gene. 1998 Mar 16;209(1-2):71-6. doi: 10.1016/s0378-1119(98)00016-x.
In comparison with the numerous Drosophila species and the mouse, the Gpdh gene in the honey bee Apis mellifera lacks most introns. This prevents the gene from producing different GPDH isoforms by alternative splicing, which occurs in Drosophila melanogaster. The sequences of the cDNA and genomic Gpdh of A. mellifera are described and show that at the amino acid level they share 84% similarity and 71% identity with D. melanogaster. The identity at the nucleotide level is 62% in the coding region, but no significant similarities were detected in the UTRs. Northern analyses revealed an accumulation of unspliced Gpdh pre-mRNA in the honey bee, probably reflecting splicing inefficiency, although it is also possible that splicing is a regulated step in Gpdh expression in A. mellifera. It is suggested that the intron loss occurred via reverse transcription of a mature Gpdh transcript.
与众多果蝇物种和小鼠相比，蜜蜂（Apis mellifera）中的Gpdh基因缺少大多数内含子。这使得该基因无法通过在黑腹果蝇中发生的可变剪接产生不同的GPDH同工型。本文描述了蜜蜂Gpdh的cDNA和基因组序列，结果显示，在氨基酸水平上，它们与黑腹果蝇的相似性为84%，一致性为71%。在编码区，核苷酸水平的一致性为62%，但在非翻译区未检测到显著的相似性。Northern分析显示，蜜蜂中存在未剪接的Gpdh前体mRNA的积累，这可能反映了剪接效率低下，尽管也有可能剪接是蜜蜂中Gpdh表达的一个受调控步骤。研究表明，内含子的丢失是通过成熟Gpdh转录本的逆转录发生的。
