Durante M, Cella L, Furusawa Y, George K, Gialanella G, Grossi G, Pugliese M, Saito M, Yang T C
Dipartimento di Scienze Fisiche, Università Federico II, Monte S. Angelo, Napoli, Italy.
Int J Radiat Biol. 1998 Mar;73(3):253-62. doi: 10.1080/095530098142347.
To measure chromosome aberrations in C3H 10T1/2 mouse fibroblasts using FISH painting at the first mitosis following exposure to 30 keV/microm hydrogen or neon ions.
Cells in plateau-phase were irradiated with 0.86 MeV protons at the TTT-3 Tandem accelerator in Naples (Italy), or with 400 MeV/n Ne ions at the HIMAC accelerator in Chiba (Japan). Colcemid-blocked cells were harvested at the first mitosis following exposure, and chromosome spreads were hybridized in situ with a fluorescein-labelled composite mouse DNA probe specific for chromosomes 2 and 8.
Protons were more efficient than neon ions at the same LET in the induction of chromosome interchanges and breaks. Yields of complex exchanges were similar for both particles at the same dose, but protons produced mostly insertions, while with Ne exposure non-reciprocal exchanges were the most frequent complex-type exchange.
Charged particles with the same LET produce different yields of chromosome aberrations, and some observed differences can be explained based on the available track-structure models.
在暴露于30 keV/μm氢或氖离子后的第一次有丝分裂时，使用荧光原位杂交（FISH）染色体描绘技术测量C3H 10T1/2小鼠成纤维细胞中的染色体畸变。
将处于平台期的细胞在意大利那不勒斯的TTT-3串联加速器上用0.86 MeV质子辐照，或在日本千叶的HIMAC加速器上用400 MeV/n氖离子辐照。在暴露后的第一次有丝分裂时收获秋水仙酰胺阻滞的细胞，并将染色体涂片与针对2号和8号染色体的荧光素标记复合小鼠DNA探针进行原位杂交。
在相同传能线密度（LET）下，质子在诱导染色体互换和断裂方面比氖离子更有效。在相同剂量下，两种粒子产生的复杂交换产率相似，但质子主要产生插入，而氖离子暴露时非相互交换是最常见的复杂型交换。
具有相同LET的带电粒子产生不同的染色体畸变产率，一些观察到的差异可以根据现有的径迹结构模型来解释。
