Matsumoto S, Yukitake H, Ohara N, Dairi T, Kanbara H, Yamada T
Nagasaki University School of Dentistry, Japan.
Microbiol Immunol. 1998;42(1):15-21. doi: 10.1111/j.1348-0421.1998.tb01964.x.
The shotgun cloning of a Mycobacterium bovis BCG (BCG) genome into pBluescript SK (+) successfully yielded a 0.9 kbp fragment, confirming the ability of Escherichia coli thyA mutant MH2702 to grow in a thymine-depleted medium. This DNA fragment contained a gene homologous to the thymidylate synthase (TS)-encoding genes (thyA) of other organisms. An inverted repeat sequence and open reading frame (ORF) were observed at the upstream region of the thyA. A computer analysis revealed that the protein encoded by this ORF possessed a structure unique for a DNA binding protein.
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