卡介苗中编码NADP依赖性乙醇脱氢酶的基因的克隆与序列分析。

Cloning and sequence analysis of the gene encoding an NADP-dependent alcohol dehydrogenase in Mycobacterium bovis BCG.

作者信息

Stélandre M, Bosseloir Y, De Bruyn J, Maes P, Content J

机构信息

Institut Pasteur du Brabant, Bruxelles, Belgium.

出版信息

Gene. 1992 Nov 2;121(1):79-86. doi: 10.1016/0378-1119(92)90164-k.

DOI:10.1016/0378-1119(92)90164-k

PMID:1427101

Abstract

The nucleotide sequence of a 1619-bp fragment of Mycobacterium bovis BCG containing the gene that encodes an alcohol dehydrogenase (ADH) has been determined. The M(r) calculated from the deduced amino acid (aa) sequence, as well as the N terminus, are in good accordance with those determined for the ADH purified from M. bovis BCG extracts. The M. bovis BCG cloned adh gene was expressed in Escherichia coli by its own promoter and the synthesized product shows ADH activity in the butane-1-ol-NADP system. Based on comparison of the aa sequence, this enzyme belongs to the zinc-containing, long-chain alcohol/polyol dehydrogenase family, which has been primarily described in eukaryotes. Of the 22 strictly conserved residues in this group, 19 are also conserved in M. bovis BCG ADH (BCGADH).

摘要

已测定了卡介苗（Mycobacterium bovis BCG）中一段1619bp的片段的核苷酸序列，该片段包含编码乙醇脱氢酶（ADH）的基因。根据推导的氨基酸（aa）序列计算出的分子量（M(r)）以及N端，与从卡介苗提取物中纯化的ADH所测定的结果高度一致。卡介苗克隆的adh基因通过其自身的启动子在大肠杆菌中表达，合成产物在丁醇-NADP系统中显示出ADH活性。基于氨基酸序列的比较，该酶属于含锌的长链醇/多元醇脱氢酶家族，该家族主要在真核生物中有所描述。在该家族中22个严格保守的残基中，有19个在卡介苗ADH（BCGADH）中也保守。