Igarashi T, Kuwata T, Yamamoto H, Moriyama H, Ui M, Miyazaki Y, Hayami M
Laboratory of Pathogenic Virus, Institute for Virus Research, Kyoto University, Japan.
Microbiol Immunol. 1998;42(1):71-4. doi: 10.1111/j.1348-0421.1998.tb01973.x.
Two SHIVs with two or three genes deleted (SHIV-drn and SHIV-dxrn) were constructed. The inoculation of monkeys with SHIV-drn resulted in short-term viremia, but inoculation with SHIV-dxrn did not. At 68 weeks post-inoculation, the monkeys were reinoculated with a 100-fold higher dose of each SHIV, but none showed viremia. Killer cell activities against HIV-1 Env were detected in the SHIV-drn- and SHIV-dxrn-inoculated monkeys. Cross-reactive killer activity against HIV-1 Gag and SIVmac Gag was observed in one monkey. Antibodies were not detected in the SHIV-dxrn-inoculated monkeys, but the SHIV-drn-inoculated monkeys showed an anamnestic antibody reaction. These data indicate that SHIV-drn is infectious to and immuno-inducible in macaques but SHIV-dxrn is not.
构建了两种缺失两个或三个基因的猿猴-人免疫缺陷病毒（SHIV-drn和SHIV-dxrn）。用SHIV-drn接种猴子导致短期病毒血症，但用SHIV-dxrn接种则未出现。接种后68周，用每种SHIV剂量高100倍的病毒再次接种猴子，但均未出现病毒血症。在接种SHIV-drn和SHIV-dxrn的猴子中检测到针对HIV-1 Env的杀伤细胞活性。在一只猴子中观察到针对HIV-1 Gag和SIVmac Gag的交叉反应杀伤活性。在接种SHIV-dxrn的猴子中未检测到抗体，但接种SHIV-drn的猴子出现了回忆性抗体反应。这些数据表明，SHIV-drn对猕猴具有感染性且可诱导免疫，但SHIV-dxrn则不然。
