一些天然和合成抗氧化剂作为β-胡萝卜素转化为维生素A的稳定剂。

Some natural and synthetic antioxidants as stabilizers of beta-carotene conversion into vitamin A.

作者信息

Gomboeva S B, Gessler N N, Shumaev K B, Khomich T I, Moiseenok A G, Bykhovskii V Y

机构信息

Bakh Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 117071 Russia.

出版信息

Biochemistry (Mosc). 1998 Feb;63(2):185-90.

PMID:9526112

Abstract

The effect of antioxidants (vitamins C and E, quercetin, probucol, butylated hydroxytoluene) on the oxidation of beta-carotene and its conversion into retinal under the influence of beta-carotene 15,15'- dioxygenase (CDO) from rat intestinal mucosa was studied. The activity of CDO decreased in the presence of oxidants. Antioxidants protected both the substrate and the enzyme. The extent of the protection depended on the antioxidant type. The combined injection of antioxidants and beta-carotene to animals completely or partially prevented the inhibition of the intestinal CDO which was caused by products of non-enzymatic oxidation of beta-carotene. Vitamins C and E, which protected the enzyme--substrate complex in vivo and in vitro, were found to be the most efficient protectors of beta-carotene conversion into retinal.

摘要

研究了抗氧化剂（维生素C和E、槲皮素、普罗布考、丁基羟基甲苯）对β-胡萝卜素氧化及其在大鼠肠黏膜β-胡萝卜素15,15'-双加氧酶（CDO）作用下转化为视黄醛的影响。在氧化剂存在的情况下，CDO的活性降低。抗氧化剂对底物和酶都有保护作用。保护程度取决于抗氧化剂的类型。向动物联合注射抗氧化剂和β-胡萝卜素可完全或部分防止由β-胡萝卜素非酶氧化产物引起的肠道CDO抑制。维生素C和E在体内和体外都能保护酶-底物复合物，被发现是β-胡萝卜素转化为视黄醛的最有效保护剂。