Isolation and characterization of site-specific endonuclease Bsp123 I from thermophilic strain Bacillus species 123.
作者信息
Alzhanova D V, Alzhanov D T, Zheleznaya L A, Matvienko N I
机构信息
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142292 Russia.
出版信息
Biochemistry (Mosc). 1998 Feb;63(2):207-11.
PMID:9526116
Abstract
The preparation of site-specific endonuclease Bsp123 I was isolated and purified from the thermophilic strain Bacillus species 123. Endonuclease Bsp123 I recognizes the sequence CGCG and cleaves it in the middle between nucleotides G and C, producing blunt ends. Thus, Bsp123 I is an isoschizomer of FnuDII. The maximal activity of the enzyme is displayed at 42 degrees C, pH 8.0, 100 mM NaCl, 10 mM MgCl2.
摘要
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