Holtorf S, Ludwig-Müller J, Apel K, Bohlmann H
Swiss Federal Institute of Technology (ETH), Institute of Plant Sciences, Zürich, Switzerland.
Plant Mol Biol. 1998 Mar;36(5):673-80. doi: 10.1023/a:1005947904830.
Viscotoxins are a group of toxic thionins found in several mistletoe species. The constitutive CaMV-omega promoter was used to drive the expression of the viscotoxin A3 cDNA from Viscum album in transgenic Arabidopsis thaliana C24. Lines with high viscotoxin A3 levels in all parts of the plant were selected and tested for resistance against the clubroot pathogen Plasmodiophora brassicae. The transgenic lines were more resistant to infection by this pathogen than the parental line.
毒肽是在几种槲寄生植物中发现的一组有毒硫堇蛋白。组成型花椰菜花叶病毒ω启动子用于驱动来自欧洲槲寄生的毒肽A3 cDNA在转基因拟南芥C24中的表达。选择植物各部分毒肽A3水平高的株系,并测试其对根肿病病原菌芸苔根肿菌的抗性。转基因株系比亲本株系对这种病原菌的感染更具抗性。