增强型绿色荧光蛋白突变体的开发与应用
Development and applications of enhanced green fluorescent protein mutants.
作者信息
Stauber R H, Horie K, Carney P, Hudson E A, Tarasova N I, Gaitanaris G A, Pavlakis G N
机构信息
National Cancer Institute-FCRDC, Frederick, MD, USA.
出版信息
Biotechniques. 1998 Mar;24(3):462-6, 468-71. doi: 10.2144/98243rr01.
The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) fluorescent protein, gave 50-fold-100-fold brighter fluorescence compared to wild-type GFP and BFP (Tyr66His), respectively, upon expression in mammalian cells. GFPsg25 and BFPsg50 have different excitation and emission maxima. This allows their use as an efficient dual-color tagging system and their independent detection in living cells.
引入多个突变导致了绿色荧光蛋白（GFP）改良突变体的产生。一种强绿色荧光蛋白（GFPsg25）和蓝色荧光蛋白（BFPsg50），在哺乳动物细胞中表达时，与野生型GFP和BFP（Tyr66His）相比，分别产生了亮50倍至100倍的荧光。GFPsg25和BFPsg50具有不同的最大激发和发射波长。这使得它们可以用作高效的双色标记系统，并在活细胞中进行独立检测。
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