Miura Y, Ozaki H S, Li T J, Uemura M, Kitano M
Department of Oral Pathology, Kagoshima University Dental School, Japan.
J Oral Pathol Med. 1998 Feb;27(2):53-8. doi: 10.1111/j.1600-0714.1998.tb02093.x.
This study was designed to establish an experimental animal model for elucidating the early stages of odontogenic cysts and tumors. It involves the in vitro treatment of tooth germs with 4-nitroquinoline 1-oxide (4NQO) at the early bell stage and their subsequent transplantation into the kidney subcapsular space. While all tooth germ transplants of the control group not exposed to the carcinogen showed continued tooth development with no pathological lesions, 21 of 23 4NQO-treated tooth germs developed into similar appearing keratinized cysts with or without associated tooth structures. The remaining two transplants failed to develop cysts and formed only a tooth. The present experimental procedure was effective in inducing keratinized cystic lesions that exhibit some similarities to human odontogenic keratocysts or primordial cysts.
本研究旨在建立一种实验动物模型，以阐明牙源性囊肿和肿瘤的早期阶段。该模型包括在钟状早期用4-硝基喹啉-1-氧化物（4NQO）对牙胚进行体外处理，随后将其移植到肾被膜下间隙。未接触致癌物的对照组所有牙胚移植均显示牙齿持续发育，无病理病变，而23个经4NQO处理的牙胚中有21个发展为外观相似的角化囊肿，伴有或不伴有相关牙齿结构。其余两个移植未形成囊肿，仅形成一颗牙齿。目前的实验方法有效地诱导了角化囊性病变，这些病变与人类牙源性角化囊肿或原始囊肿有一些相似之处。
