Trivedy C, Warnakulasuriya K A, Tavassoli M, Steingrimsdottir H, Penhallow J, Maher R, Johnson N W
RCS Department of Dental Sciences, King's College School of Medicine and Dentistry, London, England.
J Oral Pathol Med. 1998 Feb;27(2):72-7. doi: 10.1111/j.1600-0714.1998.tb02097.x.
An archival series of oral biopsies from Karachi, Pakistan, consisting of 21 cases of oral submucous fibrosis (OSF) and 27 cases of squamous cell carcinoma (SCC), of which 6 had arisen from OSF, were used to examine the aberrations in the structure and expression of the p53 tumour suppressor gene. The PCR-SSCP method was used for mutation analysis of exons 2-9, and (over)expression of p53 protein was detected by immunocytochemistry using monoclonal antibody DO 7. Positive immunostaining was observed in 15/20 (75%) of OSF specimens, 3/6 (50%) of SCC arising from OSF and 14/21 (67%) of SCC not arising from OSF. Mobility shifts in SSCP indicative of a mutation in p53 or loss of heterozygosity (deletion of a band) were seen in 13/21 cases of OSF and 15/27 cases of SCC. There was concordance between immunocytochemistry and SSCP results in a majority (33/48) of samples. Though the number of analysed SCC cases arising from OSF was limited, the results suggest that p53 mutation/protein stabilisation may play a part in the pathogenesis of OSF and its progression to SCC.
来自巴基斯坦卡拉奇的一组存档口腔活检样本，包括21例口腔黏膜下纤维化（OSF）和27例鳞状细胞癌（SCC），其中6例由OSF发展而来，用于检查p53肿瘤抑制基因的结构异常和表达情况。采用PCR - SSCP方法对第2 - 9外显子进行突变分析，并用单克隆抗体DO 7通过免疫细胞化学检测p53蛋白的（过）表达。在20份OSF样本中有15份（75%）、由OSF发展而来的6份SCC中有3份（50%）以及非由OSF发展而来的21份SCC中有14份（67%）观察到阳性免疫染色。在21份OSF病例中有13份以及27份SCC病例中有15份在SSCP中出现了指示p53突变或杂合性缺失（条带缺失）的迁移率改变。在大多数（48份样本中的33份）样本中，免疫细胞化学和SSCP结果一致。尽管分析的由OSF发展而来的SCC病例数量有限，但结果表明p53突变/蛋白稳定化可能在OSF的发病机制及其向SCC的进展中起作用。
