Heffernan J M, Eastwood S L, Nagy Z, Sanders M W, McDonald B, Harrison P J
Department of Clinical Neurology (Neuropathology), Warneford Hospital, Warneford Lane, Oxford, OX3 7JX, United Kingdom.
Exp Neurol. 1998 Apr;150(2):235-9. doi: 10.1006/exnr.1997.6772.
We measured synaptophysin mRNA in neocortical tissue from 7 prospectively assessed, pathologically verified normal individuals, 17 subjects with Alzheimer's disease (AD), and 13 subjects with a non-AD dementia. In temporal cortex (Brodmann area 21), synaptophysin mRNA was decreased in AD and non-AD dementia groups compared to controls. The loss was also present relative to polyadenylated mRNA content. Synaptophysin mRNA signal correlated negatively with the degree of dementia and negatively with the pathological severity of AD. In occipital cortex (Brodmann area 17) there were no differences between groups nor clinicopathological correlations. These data extend the evidence for a regional synaptic pathology in AD which affects synaptic protein gene expression by temporal cortex neurons.
我们检测了7名经前瞻性评估、病理证实为正常个体、17名阿尔茨海默病（AD）患者以及13名非AD痴呆患者的新皮质组织中的突触素mRNA。在颞叶皮质（布罗德曼区21），与对照组相比，AD组和非AD痴呆组的突触素mRNA减少。相对于多聚腺苷酸化mRNA含量，这种减少也存在。突触素mRNA信号与痴呆程度呈负相关，与AD的病理严重程度呈负相关。在枕叶皮质（布罗德曼区17），各组之间没有差异，也没有临床病理相关性。这些数据扩展了AD中存在区域突触病理学的证据，这种病理学影响颞叶皮质神经元的突触蛋白基因表达。
