Nagy Z, Esiri M M
OPTIMA, Department of Neuropathology and Department of Pharmacology, University of Oxford, Oxford, United Kingdom.
Exp Neurol. 1998 Apr;150(2):240-7. doi: 10.1006/exnr.1997.6753.
This study used immunocytochemistry to explore the expression of cyclins A, B, D, and E and the apoptosis-associated Bax protein in hippocampal subfields of 35 lobectomy specimens with medial temporal lobe sclerosis removed for the treatment of temporal lobe epilepsy (TLE), 2 age-matched controls, and 2 elderly patients suffering from drug-responsive epilepsy. Cyclins A and D were not detected at all in neuronal nuclei. Cyclin E was only rarely detected in neuronal nuclei in drug-controlled and TLE groups and in controls. Cyclin B was expressed in significantly more neuronal nuclei in the hippocampi in TLE than in the other groups studied. The nuclear expression of these proteins suggested that neurons had reentered the cell division cycle and reached the G2 phase. The nuclear expression of cyclin B in the hippocampus from these patients was accompanied by neuronal cytoplasmic expression of the death-related Bax protein. We interpret these neuronal findings as evidence of cell cycle disturbances and a possible apoptotic mechanism of hippocampal neuronal cell death in TLE.
本研究采用免疫细胞化学方法，探讨35例因内侧颞叶硬化行颞叶癫痫（TLE）手术切除的叶切除术标本、2例年龄匹配的对照者以及2例药物反应性癫痫老年患者海马亚区中细胞周期蛋白A、B、D和E以及凋亡相关蛋白Bax的表达情况。在神经元细胞核中完全未检测到细胞周期蛋白A和D。在药物控制组、TLE组和对照组中，仅在极少数神经元细胞核中检测到细胞周期蛋白E。与其他研究组相比，TLE组海马中细胞周期蛋白B在更多的神经元细胞核中表达。这些蛋白质的核表达表明神经元重新进入细胞分裂周期并进入G2期。这些患者海马中细胞周期蛋白B的核表达伴随着与死亡相关的Bax蛋白在神经元细胞质中的表达。我们将这些神经元研究结果解释为TLE中细胞周期紊乱以及海马神经元细胞死亡可能的凋亡机制的证据。
