采用固相萃取和反相离子对高效液相色谱法测定化妆品中的乙醇酸。

Determination of glycolic acid in cosmetic products by solid-phase extraction and reversed-phase ion-pair high-performance liquid chromatography.

作者信息

Scalia S, Callegari R, Villani S

机构信息

Dipartimento di Scienze Farmaceutiche, Università di Ferrara, Italy.

出版信息

J Chromatogr A. 1998 Feb 6;795(2):219-25. doi: 10.1016/s0021-9673(97)00973-4.

DOI:10.1016/s0021-9673(97)00973-4

PMID:9528100

Abstract

A procedure has been developed for the assay of glycolic acid in cosmetic products by reversed-phase high-performance liquid chromatography using an ion-pairing method. After dissolution in tetrahydrofuran-water (90:10, v/v), samples were purified by solid-phase extraction using silica-based strong anion-exchange cartridges and analysed directly on an Ultrasphere ODS column with UV detection at 210 nm and methanol-phosphate buffer (2:98, v/v), containing tetrabutylammonium iodide, as the mobile phase. Recovery of glycolic acid from different cosmetic matrices was between 92.4 and 96.2% and the precision of the method was better than 5.4% relative standard deviation. The procedure is rapid, simple, selective and it is suitable for routine analyses of commercial cosmetics.

摘要

已开发出一种采用离子对法的反相高效液相色谱法来测定化妆品中乙醇酸的方法。样品溶解于四氢呋喃 - 水（90:10，v/v）后，使用硅胶基强阴离子交换柱通过固相萃取进行纯化，然后直接在Ultrasphere ODS柱上进行分析，采用210 nm紫外检测，流动相为甲醇 - 磷酸盐缓冲液（2:98，v/v），其中含有碘化四丁铵。乙醇酸在不同化妆品基质中的回收率在92.4%至96.2%之间，该方法的精密度相对标准偏差优于5.4%。该方法快速、简便、具有选择性，适用于商业化妆品的常规分析。