兔肾有机阳离子转运体的分子克隆与功能表达

Molecular cloning and functional expression of a rabbit renal organic cation transporter.

作者信息

Terashita S, Dresser M J, Zhang L, Gray A T, Yost S C, Giacomini K M

机构信息

Department of Biopharmaceutical Sciences, University of California, San Francisco 94143, USA.

出版信息

Biochim Biophys Acta. 1998 Feb 2;1369(1):1-6. doi: 10.1016/s0005-2736(97)00207-1.

DOI:10.1016/s0005-2736(97)00207-1

PMID:9528667

Abstract

A cDNA encoding an organic cation transporter (rbOCT1) was isolated from rabbit kidney. The cDNA encodes a 554 amino acid protein that is highly homologous to other mammalian organic cation transporters. rbOCT1 mediated 3H-1-methyl-4-phenylpyridinium (3H-MPP+) transport in Xenopus laevis oocytes was saturable, sensitive to membrane potential, and inhibited by various organic cations. rbOCT1 mRNA transcripts are expressed in the kidney, liver, and intestine.

摘要

从兔肾中分离出一种编码有机阳离子转运体（rbOCT1）的互补DNA（cDNA）。该cDNA编码一种由554个氨基酸组成的蛋白质，它与其他哺乳动物的有机阳离子转运体高度同源。rbOCT1介导的非洲爪蟾卵母细胞中3H-1-甲基-4-苯基吡啶鎓（3H-MPP+）转运具有饱和性，对膜电位敏感，并受到各种有机阳离子的抑制。rbOCT1信使核糖核酸（mRNA）转录本在肾、肝和肠道中表达。