诺伊塞特（Noisette）的多种发育需求，它是U2 snRNP相关多肽SP3a60的果蝇同源物。
Multiple developmental requirements of noisette, the Drosophila homolog of the U2 snRNP-associated polypeptide SP3a60.
作者信息
Meyer V, Oliver B, Pauli D
机构信息
Department of Zoology and Animal Biology, University of Geneva, Switzerland.
出版信息
Mol Cell Biol. 1998 Apr;18(4):1835-43. doi: 10.1128/MCB.18.4.1835.
Abstract
We report the cloning of the noisette gene (noi), which encodes the Drosophila melanogaster ortholog of a U2 snRNP-associated splicing factor, SF3a60 (SAP61) in humans and PRP9p in Saccharomyces cerevisiae. Antibodies raised against human SF3a60 recognized NOI in flies, showing a nuclear localization in all the stages examined, including the embryo, the dividing cells of imaginal discs, and the larval polyploid nuclei. NOI is expressed in somatic and germinal cells of both male and female gonads. By mobilization of P transposons, we have generated a large number of noi mutations. Complete loss of function resulted in lethality at the end of embryogenesis, without obvious morphological defects. Hypomorphic alleles revealed multiple roles of noi for the survival and differentiation of male germ cells, the differentiation of female germ cells, and the development of several adult structures.
摘要
我们报道了诺伊塞特基因（noi）的克隆，该基因编码果蝇中与人类U2 snRNP相关剪接因子SF3a60（SAP61）以及酿酒酵母中PRP9p直系同源的蛋白。针对人类SF3a60产生的抗体在果蝇中识别出NOI，在包括胚胎、成虫盘分裂细胞和幼虫多倍体细胞核在内的所有检测阶段均显示出核定位。NOI在雄性和雌性性腺的体细胞和生殖细胞中均有表达。通过P转座子的转位，我们产生了大量的noi突变。功能完全丧失导致胚胎发育末期致死，无明显形态缺陷。亚效等位基因揭示了noi在雄性生殖细胞的存活和分化、雌性生殖细胞的分化以及几种成虫结构的发育中的多种作用。
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