Remacle A G, Noël A, Duggan C, McDermott E, O'Higgins N, Foidart J M, Duffy M J
Laboratory of Biology, University of Liège, Belgium.
Br J Cancer. 1998 Mar;77(6):926-31. doi: 10.1038/bjc.1998.153.
Matrix metalloproteinases (MMPs) are zinc dependent endopeptidases implicated in cancer invasion and metastasis. Gelatin zymography was performed on 84 human breast carcinomas and seven normal breast tissues. The precursor form of MMP-2 (72 kDa) was found in 11 (12%) samples, while its two activated forms, i.e. 62 kDa and 59 kDa, were found in three (6%) and 34 (40%) samples respectively. In contrast to MMP-2, most of the samples (52%) contained MMP-9 in its precursor form. Using ELISA, MMP-1 levels were found in 12% of the samples while MMP-3 levels were found in only 2% of the samples. Levels of MMP-2, -3 and -9 correlated inversely with numbers of nodal metastases. Neither MMP-2 nor -9 levels were significantly related to patient outcome. However, patients with high levels of a 50-kDa gelatinase band after zymography had a significantly better survival than patients with low levels. This species was never observed in normal breast tissue.
基质金属蛋白酶（MMPs）是一类锌依赖性内肽酶，与癌症侵袭和转移有关。对84例人类乳腺癌组织和7例正常乳腺组织进行了明胶酶谱分析。在11个（12%）样本中发现了MMP-2的前体形式（72 kDa），而其两种活化形式，即62 kDa和59 kDa，分别在3个（6%）和34个（40%）样本中被发现。与MMP-2不同，大多数样本（52%）含有前体形式的MMP-9。采用酶联免疫吸附测定法（ELISA），在12%的样本中检测到MMP-1水平，而在仅2%的样本中检测到MMP-3水平。MMP-2、-3和-9的水平与淋巴结转移数量呈负相关。MMP-2和-9的水平均与患者预后无显著相关性。然而，酶谱分析后出现高水平50-kDa明胶酶条带的患者比低水平患者的生存率显著更高。在正常乳腺组织中从未观察到该条带。
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