有机和无机磷酸根离子对高铁肌红蛋白电荷转移带的影响。
Effects of organic and inorganic phosphate ions on the charge-transfer band of ferric myoglobin.
作者信息
Yoshida S, Iizuka T
出版信息
Biochim Biophys Acta. 1976 Jun 15;434(2):505-8. doi: 10.1016/0005-2795(76)90241-5.
PMID:952900
Abstract
(1) Splitting of the charge-transfer band positioned at 630 nm of metmyoglobin disappeared on lowering the pH from 7.5 to 4.5 in the presence of Pi and PPi at the temperature of liquid N2. (2) The observed apparent pK value of the disappearance of the splitting depended upon the species of phosphate ions. (3) In the case of Pi, the low-temperature absorption spectra suggested that at least two phosphate ions bound to one myoglobin molecule.
摘要
(1) 在液氮温度下，于磷酸根离子（Pi）和焦磷酸根离子（PPi）存在的情况下，当pH值从7.5降至4.5时，高铁肌红蛋白位于630 nm处的电荷转移带分裂消失。(2) 观察到的分裂消失的表观pK值取决于磷酸根离子的种类。(3) 在Pi的情况下，低温吸收光谱表明至少两个磷酸根离子与一个肌红蛋白分子结合。
Zotero 插件