通过光子相关光谱法测定5型腺病毒的扩散系数和分子量
Diffusion coefficient and molecular weight of type 5 adenovirus by photon-correlation spectroscopy.
作者信息
Oliver C J, Shortridge K F, Belyavin G
出版信息
Biochim Biophys Acta. 1976 Jul 21;437(2):589-98. doi: 10.1016/0304-4165(76)90026-x.
PMID:952934
Abstract
The technique of photon-correlation spectroscopy (intensity fluctuation spectroscopy) is applied to light scatter from type 5 adenovirus undergoing Brownian motion in solution and the translation diffusion coefficient (D20,w) measured to be 0,367 +/- 0.003 Fick units. Using Svedberg's equation with previously determined parameters, a molecular weight of 165 - 10(6) +/- 5 - 10(6) is obtained.
摘要
光子相关光谱技术（强度波动光谱法）被应用于研究5型腺病毒在溶液中做布朗运动时的光散射，并测得其平移扩散系数（D20,w）为0.367±0.003菲克单位。利用斯韦德贝里方程以及先前确定的参数，得出分子量为165×10⁶±5×10⁶。
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