肌酸激酶的进一步异质性。大鼠组织中具有阴极迁移率的同工酶的存在。
The further heterogeneity of creatine kinase. Presence of isoenzymes of cathodic mobility in rat tissues.
作者信息
Sanders J L, Joung J I, Rochman H
出版信息
Biochim Biophys Acta. 1976 Jul 8;438(2):407-11. doi: 10.1016/0005-2744(76)90257-6.
Supernatant fractions from rat tissue homogenates were examined for creatine kinase (EC 2.7.3.2) isoenzymes by agarose gel electrophoresis at pH 8.6. The distribution of the various isoenzymes, and their relative contributions to the total tissue creatine kinase, is stated. A previously unreported isoenzyme associated with brain and kidney preparations is described. It is a minor contributor to brain creatine-kinase activity but is the major isoenzyme for kidney. The presence of an additional isoenzyme for cardiac tissue was confirmed; this isoenzyme exhibits similar levels of activity to that of the MB isoenzyme.
通过在pH 8.6条件下进行琼脂糖凝胶电泳，检测大鼠组织匀浆上清液中的肌酸激酶（EC 2.7.3.2）同工酶。文中阐述了各种同工酶的分布情况以及它们对组织总肌酸激酶的相对贡献。描述了一种先前未报道的与脑和肾制剂相关的同工酶。它对脑肌酸激酶活性的贡献较小，但却是肾的主要同工酶。证实了心脏组织存在另一种同工酶；这种同工酶的活性水平与MB同工酶相似。
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