Acker S, Picaud A, Duranton J
Biochim Biophys Acta. 1976 Aug 13;440(2):269-77. doi: 10.1016/0005-2728(76)90061-x.
Various photochemical activities were tested on chloroplasts of Zea mays that received 4 s of light every 4 h during the culture period. Photosystem I and Photosystem II were functioning, as well as the photosynthetic electron transport. These chloroplasts exhibited upon sodium dodecyl sulphate gel electrophoresis neither Complex 1 (Mr 70 000) generally associated with Photosystem I nor Complex 2 Mr 25 000) generally associated with Photosystem II. Chlorophyll is indeed attached to polypeptides of molecular weight 21 000 and 29 000. These results lead us to question the functional role of chloroplast protein-pigment complexes observed by sodium dodecyl sulphate gel electrophoresis.
在培养期间每4小时接受4秒光照的玉米叶绿体上测试了各种光化学活性。光系统I和光系统II以及光合电子传递均在发挥作用。这些叶绿体在十二烷基硫酸钠凝胶电泳中既未显示出通常与光系统I相关的复合物1(分子量70000),也未显示出通常与光系统II相关的复合物2(分子量25000)。叶绿素确实附着在分子量为21000和29000的多肽上。这些结果使我们质疑通过十二烷基硫酸钠凝胶电泳观察到的叶绿体蛋白质 - 色素复合物的功能作用。