Bozarth R F
Biochim Biophys Acta. 1976 Aug 2;442(1):32-6. doi: 10.1016/0005-2787(76)90172-6.
The buoyant densities in Cs2SO4 of the double-stranded RNA from the bacteriophage phi 6 and the virus-like particles of Helminthosporium maydis and Penicillium chrysogenum were determined by a Taylor series expansion and by the position and slope of the gradient relative to the isopycnic position (hinge point method). Buoyant densities for the three types of double-stranded RNA calculated by the two methods were, respectively, 1.6089 and 1.6083, 1.6065 and 1.6059 and 1.6057 and 1.6050.
通过泰勒级数展开以及相对于等密度位置的梯度位置和斜率(拐点法),测定了来自噬菌体φ6的双链RNA以及玉米大斑病菌和产黄青霉的病毒样颗粒在Cs2SO4中的浮力密度。通过这两种方法计算出的三种双链RNA的浮力密度分别为1.6089和1.6083、1.6065和1.6059以及1.6057和1.6050。