鱿鱼头部神经节中胆碱乙酰转移酶两种同工酶的制备与表征
Preparation and characterization of two isozymes of choline acetyltransferase from squid head ganglia.
作者信息
Polsky R, Shuster L
出版信息
Biochim Biophys Acta. 1976 Aug 12;445(1):25-42. doi: 10.1016/0005-2744(76)90158-3.
Two isozymes of choline acetyltransferase (Acetyl-CoA:choline O-acetyltransferase, EC 2.3.1.6) have been isolated and purified from squid head ganglia. Each isozyme contains multiple isoelectric forms with isoelectric points ranging from pH 5.0 to 6.2. The isozymes differ in their affinities for cellulose phosphate on column chromatography, as well as in their heat stabilities and in their capacities to be activated by salt. Both isozymes are stabilized by sucrose and by sulfhydryl-protecting reagents such as mercaptoethanol and dithiothreitol.
已从鱿鱼头部神经节中分离并纯化出两种胆碱乙酰转移酶同工酶（乙酰辅酶A：胆碱O-乙酰转移酶，EC 2.3.1.6）。每种同工酶都包含多个等电形式，其等电点范围为pH 5.0至6.2。这些同工酶在柱色谱上对磷酸纤维素的亲和力、热稳定性以及被盐激活的能力方面存在差异。两种同工酶都可通过蔗糖以及巯基保护试剂（如巯基乙醇和二硫苏糖醇）来稳定。
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