Slomiany B L, Piotrowski J, Piotrowski E, Slomiany A
Research Center, University of Medicine and Dentistry of New Jersey, Newark 07103-2400, USA.
Biochem Mol Biol Int. 1998 Feb;44(2):381-9. doi: 10.1080/15216549800201392.
In this study we investigated buccal mucosal cells apoptosis and the expression of regulatory cytokines, tumor necrosis factor-alpha (TNF-alpha) and interleukin-4(IL-4) with chronic ethanol ingestion. The buccal mucosa of rats maintained for 23 days on alcohol-containing and control liquid diet was assessed for IL-4 and TNF-alpha content, and the extent of epithelial cells apoptosis. While the expression of TNF-alpha in alcohol diet group showed a significant increase (1.9-fold) over that of the controls, less apparent differences between the two groups were observed in the content of IL-4 (141.8 +/- 28.2 vs. 119.8 +/- 7.3 pg/mg protein). The DNA fragmentation assays revealed that alcohol diet group also exhibited a 3.5-fold enhancement in buccal mucosal cells apoptosis. Moreover, the apoptotic index showed positive correlation (r = 0.53) with the extent of induced changes in TNF-alpha. These results demonstrate that ethanol-induced buccal mucosal cells apoptosis is triggered by the enhancement in TNF-alpha expression.
在本研究中，我们调查了慢性乙醇摄入情况下颊黏膜细胞凋亡以及调节性细胞因子肿瘤坏死因子-α（TNF-α）和白细胞介素-4（IL-4）的表达。对在含酒精液体饮食和对照液体饮食中维持23天的大鼠颊黏膜进行IL-4和TNF-α含量以及上皮细胞凋亡程度的评估。虽然酒精饮食组中TNF-α的表达相较于对照组显著增加（1.9倍），但两组之间IL-4含量的差异不太明显（分别为141.8±28.2与119.8±7.3 pg/mg蛋白质）。DNA片段化分析显示，酒精饮食组颊黏膜细胞凋亡也增强了3.5倍。此外，凋亡指数与TNF-α诱导变化程度呈正相关（r = 0.53）。这些结果表明，乙醇诱导的颊黏膜细胞凋亡是由TNF-α表达增强触发的。
