Dumur V, Dautrevaux M
Biochimie. 1976;58(5):525-32. doi: 10.1016/s0300-9084(76)80222-2.
The proteolytic specificity of thermolysin has been studied by quantitative analysis of an enzymic digest of dog myoglobin. Results confirm main specificities of thermolysin towards Phenylalanine, Isoleucine, Leucine or Tyrosine bonds; the influence of neighbourhood was also determined and the conclusions are in a good agreement with the known structure of the active site of thermolysin.
通过对犬肌红蛋白酶解产物的定量分析,研究了嗜热菌蛋白酶的蛋白水解特异性。结果证实了嗜热菌蛋白酶对苯丙氨酸、异亮氨酸、亮氨酸或酪氨酸键的主要特异性;还确定了相邻基团的影响,所得结论与嗜热菌蛋白酶活性位点的已知结构高度吻合。