Kilianska Z, Klyszejko-Stefanowicz L
Biochimie. 1976;58(6):677-80. doi: 10.1016/s0300-9084(76)80391-4.
Purified chromatin from pig lymph nodes has been prepared and used for the isolation of chromatin proteins and non-histine proteins. Saccharose nuclei from this tissue have been used for the preparation of nuclear phenol-soluble phosphoproteins. The isolated proteins have been compared by analytical electrophoresis in polyacrylamide gel in sodium dodecyl sulfate. Most chromatin non-histone proteins and nuclear phosphoproteins of pig lymph nodes have a molecular weight about 40 000 daltons and show a significant degree of homology of molecular range from 40 000 to 90 000 daltons. The phosphoproteins have been also analyzed by isoelectric focusing in polyacrylamide gel over the pH range of 3-10. They are distributed throughout the pI range of 5.8-9.0. The chromatin non-histone proteins and phosphoproteins are of acidic nature and contain 0,97 per cent tryptophan.
已制备出猪淋巴结的纯化染色质，并用于分离染色质蛋白和非组蛋白。该组织的蔗糖核已用于制备核酚溶性磷蛋白。通过在十二烷基硫酸钠聚丙烯酰胺凝胶中进行分析电泳对分离出的蛋白质进行了比较。猪淋巴结的大多数染色质非组蛋白和核磷蛋白分子量约为40000道尔顿，在40000至90000道尔顿的分子范围内显示出显著的同源性。还通过在pH值为3 - 10的聚丙烯酰胺凝胶中进行等电聚焦对磷蛋白进行了分析。它们分布在5.8至9.0的整个pI范围内。染色质非组蛋白和磷蛋白具有酸性性质，含有0.97%的色氨酸。
