Miura Y, Oda S
Department of Legal Medicine, Teikyo University School of Medicine, Tokyo, Japan.
Comp Biochem Physiol B Biochem Mol Biol. 1998 Jan;119(1):107-12. doi: 10.1016/s0305-0491(97)00289-7.
Substrate specificity and other properties of a fatty acid monooxygenase system in kidney microsomes of the Japanese house musk shrew (Suncus murinus) were examined. The suncus kidney microsomes catalyzed the hydroxylation of various saturated and unsaturated fatty acids to the omega- and (omega-1)-hydroxy derivatives. Laurate was most effectively hydroxylated among saturated and unsaturated fatty acids. The specific activity (53.79 +/- 5.59 [mean +/- SD, n = 6] nmol/nmol cytochrome P450/min) of laurate in suncus kidney microsomes was very high compared with that in liver and kidney microsomes of other species. C18 unsaturated fatty acids were converted to epoxides by a cytochrome P450-dependent fatty acid monooxygenase system in suncus kidney microsomes, in addition to omega- and (omega-1)-hydroxylation products. The monooxygenase system metabolized arachidonic acid only to omega- and (omega-1)-hydroxylation products, not to epoxidation products.
对日本麝鼩（Suncus murinus）肾微粒体中脂肪酸单加氧酶系统的底物特异性及其他特性进行了研究。麝鼩肾微粒体可将各种饱和及不饱和脂肪酸羟基化为ω-和（ω-1）-羟基衍生物。在饱和及不饱和脂肪酸中，月桂酸的羟基化效果最为显著。与其他物种的肝和肾微粒体相比，麝鼩肾微粒体中月桂酸的比活性（53.79±5.59[平均值±标准差，n = 6]nmol/ nmol细胞色素P450/分钟）非常高。除了ω-和（ω-1）-羟基化产物外，C18不饱和脂肪酸在麝鼩肾微粒体中还通过细胞色素P450依赖性脂肪酸单加氧酶系统转化为环氧化物。该单加氧酶系统仅将花生四烯酸代谢为ω-和（ω-1）-羟基化产物，而非环氧化产物。
