Dos Santos R M, Gardino P F
Programa de Neurobiologia, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.
J Neurocytol. 1998 Jan;27(1):33-43. doi: 10.1023/a:1006934919616.
A second population of tyrosine hydroxylase-immunoreactive amacrine cells was demonstrated in embryonic and adult chicken retinas by immunohistochemistry techniques in whole flat-mount preparations. The populations were differentiated on a basis of different immunostaining intensities, levels of stratification in the inner plexiform layer, and topographical distributions. Cells of one type were similar to the previously described dopaminergic amacrine cells, denoted here as tyrosine hydroxylase type 1 cells. Immunoreactive neurons of the second type observed in the present work had relatively smaller somata size, and weaker immunostaining than type 1 cells, and were located preferentially in the ventral retina. These tyrosine hydroxylase type 2 cells could be visualized from embryonic day 14 to 21 days after hatching animals. The distribution of the second population was coincident with that of the targets of centrifugal fibres and with cells involved in long proprioretinal connections. We propose that the tyrosine hydroxylase type 2 amacrine cells found in the ventral retina could mediate an important pathway to the upper half of the visual field so as to aid in the detection of predators.
通过全视网膜铺片免疫组织化学技术，在胚胎期和成年鸡视网膜中发现了第二群酪氨酸羟化酶免疫反应性无长突细胞。这些细胞群可根据不同的免疫染色强度、在内网状层的分层水平以及地形分布进行区分。其中一类细胞与先前描述的多巴胺能无长突细胞相似，在此称为酪氨酸羟化酶1型细胞。在本研究中观察到的第二类免疫反应性神经元，其胞体相对较小，免疫染色比1型细胞弱，且优先位于视网膜腹侧。这些酪氨酸羟化酶2型细胞在孵化后14天至21天的胚胎动物中均可观察到。第二群细胞的分布与离心纤维的靶标以及参与长距离视网膜内连接的细胞分布一致。我们推测，在视网膜腹侧发现的酪氨酸羟化酶2型无长突细胞可能介导了一条通向视野上半部分的重要通路，以帮助检测捕食者。
