X-537A与离子的结合。甲醇中Co(2+)的络合平衡及速率

Ion binding by X-537A. Equilibrium and rate of complexation of Co(2+) in methanol.

作者信息

Degani H, Hamilton R M, Friedman H L

出版信息

Biophys Chem. 1976 Jul;4(4):363-6. doi: 10.1016/0301-4622(76)80016-6.

Abstract

The reaction of X-537A (XH) with Co(2+) in methanol to form the complex CoX(+) has been studied fluorometrically to determine the equilibrium constant as a function of temperature. The effect of complexation on the proton NMR spectrum of the X-537A was studied to determine the kinetics of complex formation. Comparing the data for the reaction XM(+) leads to X(-) + M(2+) in methanol at 25 degrees for several M(2+) we find that the equilibrium constants increase in the order CoX(+) less than MnX(+) and span only a factor of 5 while the rate constants increase in the order NiX(+) less than CoX(+) less than MnX(+) and span a factor of more than 100.

摘要

已采用荧光分析法研究了X - 537A（XH）与甲醇中的Co(2+)反应生成配合物CoX(+)的过程，以确定平衡常数随温度的变化情况。研究了配合作用对X - 537A质子核磁共振谱的影响，以确定配合物形成的动力学。比较甲醇中几种M(2+)在25℃下反应XM(+)生成X(-) + M(2+)的数据，我们发现平衡常数按CoX(+)小于MnX(+)的顺序增加，范围仅为5倍，而速率常数按NiX(+)小于CoX(+)小于MnX(+)的顺序增加，范围超过100倍。