Carbonell G V, Alfieri A F, Alfieri A A, Vidotto M C, Levy C E, Darini A L, Yanaguita R M
Instituto de Ciências Biomédicas II, Universidade de São Paulo, Brasil.
Braz J Med Biol Res. 1997 Nov;30(11):1291-8. doi: 10.1590/s0100-879x1997001100005.
Cytotoxin production was studied in 60 Serratia marcescens strains isolated from hospitalized patients. Association of cytotoxic activity with serotype, source of isolation and presence of plasmids was also evaluated. Thirteen of the 60 S. marcescens strains produced a cytotoxic effect on Vero cells. These strains were isolated from distinct clinical sources and classified into seven different serotypes (O1:H7; O4:NM; O10:NT; O19:NM; O6,14:H4; O6,14:NM and O6,14:H1). No relationship was observed between cytotoxic activity and clinical source or serotypes of the strains. Plasmids from five cytotoxin-producing S. marcescens strains were transferred to E. coli K12/711. The transconjugants did not exhibit cytotoxicity, indicating that the cytotoxic effect is not plasmid-mediated among these strains. Although a cytotoxic activity was demonstrated in filtrates of some S. marcescens strains, further studies should be performed to assess the role of this toxin in pathogenesis.
对从住院患者中分离出的60株粘质沙雷氏菌菌株的细胞毒素产生情况进行了研究。还评估了细胞毒性活性与血清型、分离源和质粒存在之间的关联。60株粘质沙雷氏菌菌株中有13株对Vero细胞产生细胞毒性作用。这些菌株来自不同的临床来源，分为七种不同的血清型（O1:H7；O4:NM；O10:NT；O19:NM；O6,14:H4；O6,14:NM和O6,14:H1）。未观察到细胞毒性活性与菌株的临床来源或血清型之间存在关联。来自五株产生细胞毒素的粘质沙雷氏菌菌株的质粒被转移到大肠杆菌K12/711中。接合子未表现出细胞毒性，表明在这些菌株中细胞毒性作用不是由质粒介导的。尽管在一些粘质沙雷氏菌菌株的滤液中证实了细胞毒性活性，但仍应进行进一步研究以评估这种毒素在发病机制中的作用。
