Bermingham-McDonogh O, Xu Y T, Marchionni M A, Scherer S S
Cambridge NeuroScience, Massachusetts 02139, USA.
Mol Cell Neurosci. 1997;10(3-4):184-95. doi: 10.1006/mcne.1997.0654.
Neuregulins have several important functions in the development of the peripheral nervous system, acting on both developing Schwann cells and muscle fibers. To determine whether these factors are also important for peripheral nerve regeneration, we have analyzed neuregulin expression in motor and sensory neurons by Northern blots and in situ hybridization. The results of this analysis show that the predominant neuregulin isoform expressed in these neurons is a novel transmembrane splice variant. After axotomy, there is a rapid decline in neuregulin expression in both motor and sensory neurons, but following reinnervation of target tissues, neuregulin expression returns to near normal levels. These results indicate that the normal expression of neuregulins in these neurons is maintained by the interactions with target tissues.
神经调节蛋白在周围神经系统的发育中具有多种重要功能，作用于发育中的施万细胞和肌纤维。为了确定这些因子对周围神经再生是否也很重要，我们通过Northern印迹法和原位杂交分析了运动神经元和感觉神经元中神经调节蛋白的表达。该分析结果表明，在这些神经元中表达的主要神经调节蛋白异构体是一种新型跨膜剪接变体。轴突切断后，运动神经元和感觉神经元中神经调节蛋白的表达均迅速下降，但在靶组织重新神经支配后，神经调节蛋白的表达恢复到接近正常水平。这些结果表明，这些神经元中神经调节蛋白的正常表达是通过与靶组织的相互作用来维持的。
