Krishnamurthy S, Gupta V, Prasad R, Panwar S L, Prasad R
Membrane Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
FEMS Microbiol Lett. 1998 Mar 15;160(2):191-7. doi: 10.1111/j.1574-6968.1998.tb12910.x.
We have examined the expression of CDR1 (Candida drug resistance gene) in different stress conditions. There was a significant but transient enhancement of CDR1 expression associated with elevated temperatures. Most noteworthy transcriptional activation was observed with miconazole and vinblastine. Interestingly, beta-estradiol and progesterone were also able to enhance CDR1 expression. Elevated levels of CDR1 and CDR2 (a homologue of CDR1) mRNA were found in some azole-resistant clinical isolates of C. albicans. CaMDR1 (benomyl-resistant) expression, however, did not differ among all the resistant isolates. Our results confirm the existence of multiple mechanisms of azole resistance in C. albicans.
我们研究了CDR1（念珠菌耐药基因）在不同应激条件下的表达。与温度升高相关的是，CDR1表达有显著但短暂的增强。在用咪康唑和长春碱处理时观察到最显著的转录激活。有趣的是，β-雌二醇和孕酮也能够增强CDR1表达。在白色念珠菌的一些耐唑类临床分离株中发现了CDR1和CDR2（CDR1的同源物）mRNA水平升高。然而，CaMDR1（耐苯菌灵）的表达在所有耐药分离株中并无差异。我们的结果证实了白色念珠菌中存在多种唑类耐药机制。
