Kohashi M, Ohta Y, Watanabe T
School of Food and Nutritional Sciences, University of Shizuoka, Japan.
Biosci Biotechnol Biochem. 1998 Feb;62(2):297-301. doi: 10.1271/bbb.62.297.
Non-thermal effect of a ceramics radiation on glucose-6-phosphate dehydrogenase has been investigated using the enzyme, glucose-6-phosphate and NADP+ separately irradiated at 10 degrees C by a ceracompo R plate and a ceramics un-sewed cloth (sheet). The Km for glucose-6-phosphate was increased 20% after 6 h of irradiation by the plate, but the Vmax/Km was decreased 24%. After 3 h of irradiation by the sheet, the Km was increased 17%, but after 6 h of irradiation it was decreased 11%. The 3 h of irradiation by the sheet slightly increased both enthalpy and entropy changes of the reaction, but the 6 h of irradiation significantly decreased them. Both thermodynamic parameters in the activated state were increased by the sheet irradiation. The promotion energy for both formations of the enzyme-substrate and their activated complex depended on enthalpy. The different effects of two ceramics radiators on G6PDH activity were discussed.
利用陶瓷辐射的非热效应对葡萄糖 - 6 - 磷酸脱氢酶进行了研究。分别在10℃下，使用ceracompo R板和陶瓷无纺布（片）对葡萄糖 - 6 - 磷酸脱氢酶、葡萄糖 - 6 - 磷酸和NADP +进行辐照。用板辐照6小时后，葡萄糖 - 6 - 磷酸的米氏常数（Km）增加了20%，但最大反应速度与米氏常数的比值（Vmax/Km）降低了24%。用片辐照3小时后，Km增加了17%，但辐照6小时后又降低了11%。用片辐照3小时使反应的焓变和熵变略有增加，但辐照6小时则使其显著降低。片辐照使活化态的两个热力学参数均增加。酶 - 底物及其活化复合物形成的促进能均取决于焓。讨论了两种陶瓷辐射器对葡萄糖 - 6 - 磷酸脱氢酶活性的不同影响。
