Vergani P, Carballer B, Propato F, Calandria L, Targovnik H, Terzian A, Elsner B
Departamento de Patología, Hospital de Clínicas José de San Martín, Universidad de Buenos Aires.
Medicina (B Aires). 1997;57(2):143-9.
Thirty-seven nasopharyngeal carcinomas were studied obtaining the tissue from nasopharyngeal biopsies that were formalin fixed and paraffin embedded. The patients were born in Argentina, 23 men and 14 women with a mean age of 50 years. Histologically the tumors were classified as queratinizing squamous cell carcinomas, 1 case (2%); non-queratinizing squamous cell carcinomas, 15 cases (41%) and undifferentiated carcinomas, 21 cases (57%). The proliferating index (PI) was determined using monoclonal antibodies against PCNA and Ki-67 (MIB-1), resulting in 26% for PCNA and 17% for Ki-67 while no differences were found comparing PI with histological type and cases with clinical stage III and IV. The PI was of 2% in the 3 cases with clinical stage II. Immunostains for p53 were positive in 30 out of the 37 cases with no differences between the histological types, exception made for the queratinizing carcinoma which was negative. With a cut off point of 7% in the 12 cases with follow up, two groups were found with a mean survival of 35 and 12 months, a finding that was not statistically significant. Epstein-Barr virus was detected by PCR using the paraffin embedded material in 31 out of the 37 cases: 21 were undifferentiated carcinomas and 15 non-queratinizing squamous cell carcinomas; the queratinizing squamous cell carcinoma was negative. These results, published for the first time in samples from Argentinian patients are similar to those found in areas of high and low incidence of nasopharyngeal carcinomas and can be of clinical use in determining the nasopharyngeal origin of a cervical metastatic lymph node of an unknown primary.
对37例鼻咽癌患者进行了研究，其组织取自经福尔马林固定、石蜡包埋的鼻咽活检标本。这些患者出生于阿根廷，男性23例，女性14例，平均年龄50岁。组织学上，肿瘤分类为角化性鳞状细胞癌1例（2%）；非角化性鳞状细胞癌15例（41%）；未分化癌21例（57%）。使用抗PCNA和Ki-67（MIB-1）的单克隆抗体测定增殖指数（PI），PCNA为26%，Ki-67为17%，PI与组织学类型以及临床III期和IV期病例相比无差异。临床II期的3例患者PI为2%。37例中有30例p53免疫染色呈阳性，各组织学类型之间无差异，但角化性癌为阴性。在12例有随访的患者中，以7%为界值分为两组，平均生存期分别为35个月和12个月，这一结果无统计学意义。使用石蜡包埋材料通过PCR在37例中的31例检测到爱泼斯坦-巴尔病毒：21例为未分化癌，15例为非角化性鳞状细胞癌；角化性鳞状细胞癌为阴性。这些首次发表于阿根廷患者样本的结果与在鼻咽癌高发病率和低发病率地区所发现的结果相似，在确定不明原发灶的颈部转移性淋巴结的鼻咽来源方面具有临床应用价值。
