Molloy J B, Eaves F W, Jeston P J, Minchin C M, Stewart N P, Lew A E, Jorgensen W K
Queensland Department of Primary Industries, Animal Research Institute, Yeerongpilly, Australia.
Avian Dis. 1998 Jan-Mar;42(1):119-23.
A polymerase chain reaction (PCR)-based assay was developed for the detection of Eimeria acervulina. Primers were designed to amplify a fragment of the EASZ240/160 sporozoite antigen gene. The PCR assay detected as few as 10 E. acervulina oocysts in a mixed population containing a total of 10(6) oocysts. No nonspecific reaction was observed with any other species of avian Eimeria known to occur in Australia. PCR products from genomic DNA were 237 bp larger than predicted from previously reported cDNA sequences. Sequencing of the product revealed the presence of a probable intron. This work demonstrates the potential of PCR-based assays for identification and detection of avian Eimeria. Potential uses include identification of minor species present in mixed infections and quality control in the production of live vaccines.
开发了一种基于聚合酶链反应（PCR）的检测方法用于检测堆型艾美耳球虫。设计引物以扩增EASZ240/160子孢子抗原基因的一个片段。该PCR检测方法在总共含有10⁶个卵囊的混合群体中能检测到低至10个堆型艾美耳球虫卵囊。对于已知在澳大利亚出现的任何其他禽类艾美耳球虫物种均未观察到非特异性反应。来自基因组DNA的PCR产物比先前报道的cDNA序列预测的大237bp。产物测序显示存在一个可能的内含子。这项工作证明了基于PCR的检测方法在禽类艾美耳球虫鉴定和检测方面的潜力。潜在用途包括鉴定混合感染中存在的次要物种以及活疫苗生产中的质量控制。
