Renjifo X, Howard C, Kerkhofs P, Denis M, Urbain J, Moser M, Pastoret P P
Laboratoire de Physiologie Animale, Université Libre de Bruxelles, Rhode Saint Genese, Belgium.
Vet Immunol Immunopathol. 1997 Dec 12;60(1-2):77-88. doi: 10.1016/s0165-2427(97)00092-5.
Optimal activation of T lymphocytes depends on TCR interaction with peptide/MHC complexes in conjunction with costimulatory signals, which are delivered by specialized cells called antigen-presenting-cells (APC). The population of APC is heterogeneous and includes dendritic cells, B cells and macrophages. The family of dendritic cells (DC) is widely distributed in tissues and plays a major role in the induction of primary T-dependent immune responses. The aim of this paper was to isolate and characterize dendritic cells from cattle. Two methods are described that have been used to isolate dendritic cells from bovine peripheral blood. One method involves sequential depletion of other cells, adherence and isolation of low buoyant density cells on Metrizamide column. The second involves enrichment of cells displaying receptors for plasma fibronectin, followed by adherence and separation on Metrizamide. Both preparations were characterized morphologically by flow cytometry and functionally. Both procedures produced enriched populations that did not express molecules typical of T cells (CD3, CD4, CD8, WC1), B cells (sIg, CD21) and monocytes (CD14, Fc gamma 2R). Procedure 2 yielded cells with a typical veiled DC morphology that were highly effective at stimulating allogeneic T cells. Procedure 1 yielded cells that did not have the veiled morphology and were less effective in the MLR which may represent a more immature stage.
T淋巴细胞的最佳激活取决于T细胞受体与肽/MHC复合物的相互作用以及共刺激信号，这些信号由称为抗原呈递细胞（APC）的特殊细胞传递。APC群体是异质性的，包括树突状细胞、B细胞和巨噬细胞。树突状细胞（DC）家族广泛分布于组织中，在原发性T细胞依赖性免疫反应的诱导中起主要作用。本文的目的是从牛中分离和鉴定树突状细胞。描述了两种用于从牛外周血中分离树突状细胞的方法。一种方法包括依次去除其他细胞、贴壁以及在Metrizamide柱上分离低浮力密度细胞。第二种方法包括富集显示血浆纤连蛋白受体的细胞，然后贴壁并在Metrizamide上分离。两种制备物都通过流式细胞术进行形态学和功能表征。两种方法都产生了富集的细胞群体，这些细胞不表达T细胞（CD3、CD4、CD8、WC1）、B细胞（sIg、CD21）和单核细胞（CD14、Fcγ2R）的典型分子。方法2产生的细胞具有典型的面纱样DC形态，在刺激同种异体T细胞方面非常有效。方法1产生的细胞没有面纱样形态，在混合淋巴细胞反应中效果较差，这可能代表了一个更不成熟的阶段。
