Adler V V, Ioannesiants I A, Kulevich E E, Kadagidze Z G, Shapot V S
Biull Eksp Biol Med. 1976 Jul;82(7):811-4.
Biphasic character of dexamethasone action on the lymphoid tissue is observed in the tissue culture of partially purified peripheral blood lymphocytes. Stimulation of the RNA synthesis occurred after 6-hour incubation of lymphocytes with the hormone. Sedimentation analysis of labeled RNA, fractionated in a column containing poly-U sepharose pointed to increase in the quantity of the messenger RNA and enrichment of the cytoplasmic RNA with poly-A sequences. At the same time the Mn++-dependable RNA polymerase sensitive to alpha-amanitin was activated. Inhibition of the RNA synthesis is observed after 24-hour cultivation of lymphocytes with the hormone. Biphasic effect of the steroid is also seen in the reaction of rosetteformation.
在部分纯化的外周血淋巴细胞的组织培养中观察到地塞米松对淋巴组织作用的双相特征。淋巴细胞与该激素孵育6小时后，RNA合成受到刺激。在含有聚-U琼脂糖的柱中分级分离的标记RNA的沉降分析表明，信使RNA的数量增加，并且细胞质RNA富含聚-A序列。同时，对α-鹅膏蕈碱敏感的Mn++依赖性RNA聚合酶被激活。淋巴细胞与该激素培养24小时后，观察到RNA合成受到抑制。在玫瑰花结形成反应中也可见到该类固醇的双相效应。
