Ozawa M, Oki Y, Watanabe F, Iino K, Masuzawa M, Iwabuchi M, Yoshimi T
Department of Medicine, Hamamatsu University School of Medicine, Japan.
Peptides. 1998;19(3):513-8. doi: 10.1016/s0196-9781(97)00464-6.
We examined the effect of urocortin (Ucn) on the adrenocorticotropin (ACTH) release from cultured rat anterior pituitary cells and AtT 20 cells. Synthetic rat (r)Ucn was not soluble in 0.1 N HCl but soluble in alkaline solvents with diminished corticotropin-releasing activity. rUcn dissolved in 0.1 M sodium phosphate buffer as a stock solution maintained its bioactivity and had the equal corticotropin-releasing activity with rat/human corticotropin-releasing factor (r/hCRF). rUcn stimulated the adrenocorticotropin release via CRF-receptors accompanied by the additive effect with r/hCRF, the synergistic effect with arginine vasopressin and the dose-dependent inhibition of a potent CRF-receptor antagonist.
我们研究了尿皮质素（Ucn）对培养的大鼠垂体前叶细胞和AtT 20细胞促肾上腺皮质激素（ACTH）释放的影响。合成的大鼠（r）Ucn不溶于0.1 N盐酸，但可溶于具有降低促肾上腺皮质激素释放活性的碱性溶剂中。溶解于0.1 M磷酸钠缓冲液中的rUcn储备液保持其生物活性，并且与大鼠/人类促肾上腺皮质激素释放因子（r/hCRF）具有同等的促肾上腺皮质激素释放活性。rUcn通过CRF受体刺激促肾上腺皮质激素释放，伴有与r/hCRF的相加作用、与精氨酸加压素的协同作用以及对强效CRF受体拮抗剂的剂量依赖性抑制作用。
