尿皮质素的作用及其与促肾上腺皮质激素（ACTH）促分泌素的相互作用对ACTH释放的影响。

Effect of urocortin and its interaction with adrenocorticotropin (ACTH) secretagogues on ACTH release.

作者信息

Ozawa M, Oki Y, Watanabe F, Iino K, Masuzawa M, Iwabuchi M, Yoshimi T

机构信息

Department of Medicine, Hamamatsu University School of Medicine, Japan.

出版信息

Peptides. 1998;19(3):513-8. doi: 10.1016/s0196-9781(97)00464-6.

DOI:10.1016/s0196-9781(97)00464-6

PMID:9533639

Abstract

We examined the effect of urocortin (Ucn) on the adrenocorticotropin (ACTH) release from cultured rat anterior pituitary cells and AtT 20 cells. Synthetic rat (r)Ucn was not soluble in 0.1 N HCl but soluble in alkaline solvents with diminished corticotropin-releasing activity. rUcn dissolved in 0.1 M sodium phosphate buffer as a stock solution maintained its bioactivity and had the equal corticotropin-releasing activity with rat/human corticotropin-releasing factor (r/hCRF). rUcn stimulated the adrenocorticotropin release via CRF-receptors accompanied by the additive effect with r/hCRF, the synergistic effect with arginine vasopressin and the dose-dependent inhibition of a potent CRF-receptor antagonist.

摘要

我们研究了尿皮质素（Ucn）对培养的大鼠垂体前叶细胞和AtT 20细胞促肾上腺皮质激素（ACTH）释放的影响。合成的大鼠（r）Ucn不溶于0.1 N盐酸，但可溶于具有降低促肾上腺皮质激素释放活性的碱性溶剂中。溶解于0.1 M磷酸钠缓冲液中的rUcn储备液保持其生物活性，并且与大鼠/人类促肾上腺皮质激素释放因子（r/hCRF）具有同等的促肾上腺皮质激素释放活性。rUcn通过CRF受体刺激促肾上腺皮质激素释放，伴有与r/hCRF的相加作用、与精氨酸加压素的协同作用以及对强效CRF受体拮抗剂的剂量依赖性抑制作用。