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Mammalian cytosolic chaperonin.

作者信息

Cowan N J

机构信息

Department of Biochemistry, New York University School of Medicine, New York 10016-6402, USA.

出版信息

Methods Enzymol. 1998;290:230-41. doi: 10.1016/s0076-6879(98)90022-2.

DOI:10.1016/s0076-6879(98)90022-2
PMID:9534166
Abstract

Cytosolic chaperonin, the eukaryotic cytosolic homolog of GroEL, has certain unusual features that make it uniquely useful for studying the mechanism of chaperonin action. It is of particular interest as an essential component in the generation of native actin and tubulin in vivo. We describe a method for the purification of mammalian c-cpn from rabbit reticulocyte lysate via a three-step procedure involving ion-exchange chromatography, affinity selection on ATP-agarose, and gel filtration. We also describe a sensitive in vitro-folding assay for the activity of c-cpn and other chaperone proteins, and a simple nondenaturing gel assay for the analysis of folding reaction products.

摘要

相似文献

1
Mammalian cytosolic chaperonin.
Methods Enzymol. 1998;290:230-41. doi: 10.1016/s0076-6879(98)90022-2.
2
Chaperonin-mediated folding in the eukaryotic cytosol proceeds through rounds of release of native and nonnative forms.伴侣蛋白介导的真核细胞质中的折叠过程通过天然和非天然形式的一轮轮释放来进行。
Cell. 1997 Jun 13;89(6):927-37. doi: 10.1016/s0092-8674(00)80278-0.
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Structure and function of a protein folding machine: the eukaryotic cytosolic chaperonin CCT.一种蛋白质折叠机器的结构与功能：真核细胞胞质伴侣蛋白CCT
FEBS Lett. 2002 Oct 2;529(1):11-6. doi: 10.1016/s0014-5793(02)03180-0.
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Eukaryotic cytosolic chaperonin contains t-complex polypeptide 1 and seven related subunits.真核细胞胞质伴侣蛋白包含t-复合体多肽1和七个相关亚基。
Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11975-9. doi: 10.1073/pnas.90.24.11975.
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Prefoldin, a chaperone that delivers unfolded proteins to cytosolic chaperonin.前折叠素，一种将未折叠蛋白质递送至胞质伴侣蛋白的分子伴侣。
Cell. 1998 May 29;93(5):863-73. doi: 10.1016/s0092-8674(00)81446-4.
6
The substrate recognition mechanisms in chaperonins.伴侣蛋白中的底物识别机制。
J Mol Recognit. 2004 Mar-Apr;17(2):85-94. doi: 10.1002/jmr.654.
7
Partial occlusion of both cavities of the eukaryotic chaperonin with antibody has no effect upon the rates of beta-actin or alpha-tubulin folding.真核伴侣蛋白两个腔室的部分封闭对β-肌动蛋白或α-微管蛋白的折叠速率没有影响。
J Biol Chem. 2000 Feb 18;275(7):4587-91. doi: 10.1074/jbc.275.7.4587.
8
MgATP binding to the nucleotide-binding domains of the eukaryotic cytoplasmic chaperonin induces conformational changes in the putative substrate-binding domains.MgATP与真核细胞质伴侣蛋白的核苷酸结合结构域结合会诱导假定的底物结合结构域发生构象变化。
Protein Sci. 1998 Jul;7(7):1524-30. doi: 10.1002/pro.5560070705.
9
The 'sequential allosteric ring' mechanism in the eukaryotic chaperonin-assisted folding of actin and tubulin.真核伴侣蛋白协助肌动蛋白和微管蛋白折叠中的“顺序变构环”机制。
EMBO J. 2001 Aug 1;20(15):4065-75. doi: 10.1093/emboj/20.15.4065.
10
Mutational screen identifies critical amino acid residues of beta-actin mediating interaction between its folding intermediates and eukaryotic cytosolic chaperonin CCT.突变筛选鉴定出β-肌动蛋白的关键氨基酸残基，这些残基介导其折叠中间体与真核细胞溶质伴侣蛋白CCT之间的相互作用。
J Struct Biol. 2001 Aug;135(2):185-97. doi: 10.1006/jsbi.2001.4389.

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Functional interaction between phosducin-like protein 2 and cytosolic chaperonin is essential for cytoskeletal protein function and cell cycle progression.
视紫红质样蛋白2与胞质伴侣蛋白之间的功能相互作用对于细胞骨架蛋白功能和细胞周期进程至关重要。
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The yeast ubiquitin protease, Ubp3p, promotes protein stability.酵母泛素蛋白酶Ubp3p可促进蛋白质稳定性。
Genetics. 2002 Nov;162(3):1079-89. doi: 10.1093/genetics/162.3.1079.