Seale J W, Brazil B T, Horowitz P M
Department of Biochemistry, University of Texas Health Science Center at San Antonio 78284-7760, USA.
Methods Enzymol. 1998;290:318-23. doi: 10.1016/s0076-6879(98)90028-3.
We have elucidated conditions for the covalent incorporation of a nonspecific hydrophobic probe, bisANS, into various proteins. Using this method, we are able to map hydrophobic surfaces in proteins. In addition, we have shown that for GroEL, we are able to use the fluorescence of the incorporated bisANS to monitor conformational changes in a defined region of the protein in response to various effectors. This method should be useful for studying both protein structure and dynamics.
我们已经阐明了将非特异性疏水探针双-ANS共价掺入各种蛋白质的条件。利用这种方法，我们能够绘制蛋白质中的疏水表面。此外，我们已经表明，对于GroEL，我们能够利用掺入的双-ANS的荧光来监测蛋白质特定区域响应各种效应物的构象变化。这种方法对于研究蛋白质结构和动力学都应该是有用的。
