Torres A R, Peterson E A
J Biochem Biophys Methods. 1979 Dec;1(6):349-60. doi: 10.1016/0165-022x(79)90026-5.
Simple mixtures of proteins have been used as models to demonstrate that the fractionation of proteins by carboxymethyldextrans on an anion exchanger (Peterson, E.A. (1978) Anal. Biochem. 90, 767--784) is fundamentally a displacement process. Examples include the separation of two closely similar proteins, the A and B forms of beta-lactoglobulin. The use of carboxymethyldextran spacers was essential for good separation, since resolution based on protein--protein displacement was generally inadequate. The origins and effects of heterogeneity in the preparations used as spacers are discussed.
蛋白质的简单混合物已被用作模型，以证明羧甲基葡聚糖在阴离子交换剂上对蛋白质的分级分离（彼得森，E.A.（1978年）《分析生物化学》90卷，767 - 784页）从根本上来说是一个置换过程。例子包括两种非常相似的蛋白质，即β - 乳球蛋白的A和B两种形式的分离。使用羧甲基葡聚糖间隔物对于良好的分离至关重要，因为基于蛋白质 - 蛋白质置换的分辨率通常不足。本文讨论了用作间隔物的制剂中异质性的起源和影响。
