Mikloska Z, Danis V A, Adams S, Lloyd A R, Adrian D L, Cunningham A L
Centre for Virus Research, Westmead Institutes of Health Research, NSW, Australia.
J Infect Dis. 1998 Apr;177(4):827-38. doi: 10.1086/515236.
Recurrent human herpes simplex lesions are infiltrated by macrophages and CD4 and CD8 lymphocytes, which secrete cytokines and chemokines. Vesicle fluid was examined by ELISA for the presence of cytokines and beta (C-C) chemokines. On the first day of the lesion, high concentrations of interleukin (IL)-1beta, and IL-6, moderate concentrations of IL-1alpha and IL-10, and low concentrations of IL-12 and beta chemokines were found; levels of macrophage inflammatory protein (MIP)-1beta were significantly higher than levels of MIP-1alpha and RANTES. At day 3, the concentrations of IL-1beta, IL-6, and MIP-1beta were lower, whereas the levels of IL-10, IL-12, and MIP-1alpha remained similar, and the level of tumor necrosis factor-alpha was now detectable. Herpes simplex virus infection of keratinocytes in vitro stimulated production of beta chemokines followed by IL-12 and then IL-10, IL-1alpha, IL-1beta, and IL-6, indicating a potential role for these events in early recruitment, activation, and interferon-gamma production of CD4 cells in herpetic lesions.
复发性人类单纯疱疹皮损中有巨噬细胞、CD4和CD8淋巴细胞浸润，这些细胞会分泌细胞因子和趋化因子。通过酶联免疫吸附测定法检测水疱液中细胞因子和β（C-C）趋化因子的存在情况。在皮损出现的第一天，发现白细胞介素（IL）-1β、IL-6浓度高，IL-1α和IL-10浓度中等，IL-12和β趋化因子浓度低；巨噬细胞炎性蛋白（MIP）-1β的水平显著高于MIP-1α和调节激活正常T细胞表达和分泌因子（RANTES）的水平。在第3天，IL-1β、IL-6和MIP-1β的浓度降低，而IL-10、IL-12和MIP-1α的水平保持相似，此时可检测到肿瘤坏死因子-α的水平。体外角质形成细胞的单纯疱疹病毒感染刺激β趋化因子的产生，随后是IL-12，然后是IL-10、IL-1α、IL-1β和IL-6，这表明这些事件在疱疹皮损中CD4细胞的早期募集、激活和γ干扰素产生中可能发挥作用。
