Campbell E A, Choi S Y, Masure H R
Laboratory of Molecular Infectious Diseases, Rockefeller University, New York, NY 10021, USA.
Mol Microbiol. 1998 Mar;27(5):929-39. doi: 10.1046/j.1365-2958.1998.00737.x.
Transformation in bacteria is the uptake and incorporation of exogenous DNA into a cell's genome. Several species transform naturally during a regulated state defined as competence. Genetic elements in Streptococcus pneumoniae induced during transformation were identified by combining a genetic screen with genomic analysis. Six loci were discovered that composed a competence-induced regulon. These loci shared a consensus promoter sequence and encoded proteins, some of which were similar to proteins involved in DNA processing during transformation in other bacteria. Each locus was induced during competence and essential for genetic transformation.
细菌中的转化是指外源DNA被摄取并整合到细胞基因组中。几种细菌在一种被定义为感受态的调控状态下自然发生转化。通过将遗传筛选与基因组分析相结合，鉴定出了肺炎链球菌在转化过程中诱导产生的遗传元件。发现了六个位点，它们构成了一个感受态诱导的操纵子。这些位点共享一个共有启动子序列并编码蛋白质，其中一些蛋白质与其他细菌转化过程中参与DNA处理的蛋白质相似。每个位点在感受态期间被诱导，并且对遗传转化至关重要。
