一氧化氮不参与辛伐他汀诱导的PC12细胞的细胞分裂和分化。

NO is not involved in the simvastatin induced cell division and differentiation in PC12 cells.

作者信息

Sano M, Sato-Suzuki I, Fujita H, Morita I, Nagao M, Murota S

机构信息

Department of Physiological Chemistry, Graduate School, Tokyo Medical and Dental University, Japan.

出版信息

Neurosci Lett. 1998 Feb 27;243(1-3):73-6. doi: 10.1016/s0304-3940(98)00086-x.

DOI:10.1016/s0304-3940(98)00086-x

PMID:9535116

Abstract

Simvastatin, a potent 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor has been reported to inhibit cell division and induce neurite-like outgrowth in PC12 cells [Sato-Suzuki, I. and Murota, S., Neurosci. Lett., 220 (1996) 21-24]. In the present paper, we examined whether the induced nitric oxide (NO) in the simvastatin-treated PC12 cells is involved in the growth arrest and differentiation as reported in nerve growth factor (NGF) treated PC12 cells. Treatment of PC12 cells with simvastatin caused peripherin formation and enhanced NO production just like NGF-treated PC12 cells. Different from NGF, however, NO synthase inhibitors could not affect the growth arrest and differentiation in simvastatin-treated PC12 cells. In conclusion, NO had nothing to do with cell division and differentiation in simvastatin-treated PC12 cells.

摘要

辛伐他汀是一种强效的3-羟基-3-甲基戊二酰辅酶A（HMG-CoA）还原酶抑制剂，据报道它能抑制PC12细胞的细胞分裂并诱导其长出神经突样突起[Sato-Suzuki, I.和Murota, S., 《神经科学快报》, 220 (1996) 21 - 24]。在本文中，我们研究了辛伐他汀处理的PC12细胞中诱导产生的一氧化氮（NO）是否如神经生长因子（NGF）处理的PC12细胞那样参与生长停滞和分化过程。用辛伐他汀处理PC12细胞会导致外周蛋白形成并增强NO生成，这与NGF处理的PC12细胞情况相同。然而，与NGF不同的是，NO合酶抑制剂不会影响辛伐他汀处理的PC12细胞的生长停滞和分化。总之，在辛伐他汀处理的PC12细胞中，NO与细胞分裂和分化无关。