一种与性别相关的Ace基因，与致倦库蚊中不敏感的乙酰胆碱酯酶介导的杀虫剂抗性无关。
A sex-linked Ace gene, not linked to insensitive acetylcholinesterase-mediated insecticide resistance in Culex pipiens.
作者信息
Malcolm C A, Bourguet D, Ascolillo A, Rooker S J, Garvey C F, Hall L M, Pasteur N, Raymond M
机构信息
School of Biological Sciences, Queen Mary and Westfield College, University of London, UK.
出版信息
Insect Mol Biol. 1998 May;7(2):107-20. doi: 10.1046/j.1365-2583.1998.72055.x.
An acetylcholinesterase (AChE) gene, Ace.x, showing 93% identity of deduced amino acid sequence to Anopheles stephensi Ace has been cloned from a Culex pipiens strain homozygous for insensitive AChE (iAChE) mediated insecticide resistance. DNA sequence of genomic DNA clones identified exons 2-5. RFLP of six clones indicated four possible alleles. Linkage analysis located Ace.x to chromosome I, less than 0.8 centimorgans from the sex locus, whereas the locus conferring resistance was 2.0 centimorgans from plum-eye on chromosome II. Ace.1 coding for AChE1, which is associated with resistance, is therefore autosomal. We propose that Ace.x is the recently postulated Ace.2 coding for the biochemically distinct AChE2, which is not associated with resistance.
从对不敏感乙酰胆碱酯酶（iAChE）介导的杀虫剂抗性呈纯合状态的致倦库蚊品系中克隆出了一个乙酰胆碱酯酶（AChE）基因Ace.x，其推导氨基酸序列与斯氏按蚊Ace的一致性为93%。基因组DNA克隆的DNA序列鉴定出了外显子2至5。六个克隆的限制性片段长度多态性（RFLP）表明存在四种可能的等位基因。连锁分析将Ace.x定位到了I号染色体上，距离性位点不到0.8厘摩，而赋予抗性的位点在II号染色体上距离李子眼为2.0厘摩。因此，编码与抗性相关的乙酰胆碱酯酶1（AChE1）的Ace.1是常染色体的。我们推测Ace.x是最近假定的编码生化特性不同的乙酰胆碱酯酶2（AChE2）的Ace.2，而AChE2与抗性无关。
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