通过毛细管区带电泳法测定转化天仙子植株中的主要托烷生物碱。

Determination of the main tropane alkaloids from transformed Hyoscyamus muticus plants by capillary zone electrophoresis.

作者信息

Eeva M, Salo J P, Oksman-Caldentey K M

机构信息

Department of Pharmacy, University of Helsinki, Finland.

出版信息

J Pharm Biomed Anal. 1998 Jan;16(5):717-22. doi: 10.1016/s0731-7085(97)00121-0.

DOI:10.1016/s0731-7085(97)00121-0

PMID:9535182

Abstract

A capillary zone electrophoretic method (CZE) was developed using an uncoated fused silica capillary for the separation and determination of the main tropane alkaloids. The applicability of the developed method for analysis of plant samples was examined by analyzing samples of transgenic Egyptian henbane Hyoscyamus muticus (L.) plants. A simple 40 mM phosphate buffer at pH 7.8 using a voltage of 20 kV was found the best for this purpose. The main tropane alkaloids, atropine and scopolamine as well as nor-(-)-scopolamine, and tropic acid, the precursor of tropane alkaloids, could be separated in less than 13 min. The linear concentration range for atropine was 5.00-140 microg ml(-1), for scopolamine 7.50-210 microg ml(-1) and for tropic acid 2.50-70.0 microg ml(-1).

摘要

开发了一种毛细管区带电泳法（CZE），使用未涂层熔融石英毛细管分离和测定主要的托烷生物碱。通过分析转基因埃及天仙子（Hyoscyamus muticus (L.)）植物的样品，检验了所开发方法对植物样品分析的适用性。发现使用20 kV电压、pH 7.8的简单40 mM磷酸盐缓冲液最适合此目的。主要的托烷生物碱阿托品、东莨菪碱以及去甲-(-)-东莨菪碱和托烷生物碱的前体托品酸，可在不到13分钟内分离。阿托品的线性浓度范围为5.00-140 μg ml(-1)，东莨菪碱为7.50-210 μg ml(-1)，托品酸为2.50-70.0 μg ml(-1)。