Rocha J B, Landeira-Fernandez A M, de Meis L
Departamento de Quimica, Universidade Federal de Santa Maria, Brazil.
Biochem Biophys Res Commun. 1998 Mar 27;244(3):720-3. doi: 10.1006/bbrc.1998.8160.
The effects of heparin and dextran sulfate 8,000 on two isoforms of the sarco/endoplasmic reticulum Ca(2+)-ATPase of different animal tissues and on the corn root H(+)-ATPase were examined. In the absence of sulfated polysaccharides the pH profile's of the three transport ATPases were quite different, but after the addition of heparin or dextran sulfate 8,000 the pH profiles of the three enzymes became similar, all showed maximal activity at pH 7.0. Potassium and sodium antagonized the effects of sulfated polysaccharides on the three transport ATPases, but the antagonism was considerably reduced at acidic pH values.
研究了肝素和8000硫酸葡聚糖对不同动物组织的肌浆/内质网Ca(2+)-ATP酶的两种同工型以及玉米根H(+)-ATP酶的影响。在没有硫酸化多糖的情况下，三种转运ATP酶的pH曲线差异很大，但加入肝素或8000硫酸葡聚糖后，三种酶的pH曲线变得相似，均在pH 7.0时显示最大活性。钾和钠拮抗硫酸化多糖对三种转运ATP酶的作用，但在酸性pH值下这种拮抗作用明显减弱。
