King P, Goodbourn S
Division of Biochemistry, Department of Cellular and Molecular Sciences, St. George's Hospital Medical School, University of London, London SW17 0RE, United Kingdom.
J Biol Chem. 1998 Apr 10;273(15):8699-704. doi: 10.1074/jbc.273.15.8699.
Apoptosis involves the activation of a cascade of interleukin-1beta converting enzyme-like proteases (caspases), a group of cysteine proteases related to the prototype interleukin-1beta-converting enzyme (caspase-1). These proteases cleave specific intracellular targets such as poly(ADP-ribose) polymerase, DNA-dependent protein kinase, and nuclear lamins. We show here that apoptosis can be induced by double-stranded RNA. The induction of apoptosis by double-stranded RNA and other agents leads to the cleavage by a caspase of the signal transducer and activator of transcription factor, STAT1 which is pivotal in the signal transduction pathways of the interferons and many other cytokines and growth factors. The product of this cleavage is no longer able to mediate interferon-activated signal transduction and the cleavage event may play a role in regulating the apoptosis response itself.
细胞凋亡涉及一系列白细胞介素 - 1β转化酶样蛋白酶（半胱天冬酶）的激活，这是一组与原型白细胞介素 - 1β转化酶（半胱天冬酶 - 1）相关的半胱氨酸蛋白酶。这些蛋白酶可切割特定的细胞内靶点，如聚（ADP - 核糖）聚合酶、DNA依赖性蛋白激酶和核纤层蛋白。我们在此表明，双链RNA可诱导细胞凋亡。双链RNA和其他因子诱导的细胞凋亡会导致半胱天冬酶对信号转导和转录激活因子STAT1的切割，STAT1在干扰素以及许多其他细胞因子和生长因子的信号转导途径中起关键作用。这种切割产物不再能够介导干扰素激活的信号转导，并且切割事件可能在调节细胞凋亡反应本身中发挥作用。
