碱性磷酸钙晶体可诱导人成纤维细胞合成并分泌92 kDa明胶酶（明胶酶B/基质金属蛋白酶9）。
Basic calcium phosphate crystals induce synthesis and secretion of 92 kDa gelatinase (gelatinase B/matrix metalloprotease 9) in human fibroblasts.
作者信息
McCarthy G M, Macius A M, Christopherson P A, Ryan L M, Pourmotabbed T
机构信息
Department of Medicine, Medical College of Wisconsin, Milwaukee, USA.
出版信息
Ann Rheum Dis. 1998 Jan;57(1):56-60. doi: 10.1136/ard.57.1.56.
OBJECTIVE
Synovial fluid basic calcium phosphate (BCP) crystals are associated with severe joint degeneration accompanied by synovial hypertrophy. The metalloprotease 92 kDa gelatinase (MMP-9) has been implicated in the degradation of extracellular matrix in osteoarthritis, but the ability of BCP crystals to induce gelatinase in human fibroblasts or in adult porcine chondrocytes has not previously been studied. The hypothesis that the mitogenic response to BCP crystals is accompanied by induction and secretion of MMP-9 was studied.
METHODS
MMP-9 messenger RNA (mRNA) was detected by northern blot and reverse transcription-polymerase chain reaction (RT-PCR). Gelatinase secretion was identified by western blot and zymography of conditioned media.
RESULTS
BCP crystals caused a concentration dependent induction of MMP-9 mRNA accumulation and protein secretion in human fibroblasts but not in adult porcine chondrocytes.
CONCLUSION
BCP crystals induce MMP-9 production by HF but not adult porcine chondrocytes. Fibroblast MMP-9 may be an important mediator of the joint destruction associated with synovial fluid BCP crystals.
目的
滑液碱性磷酸钙（BCP）晶体与伴有滑膜肥大的严重关节退变相关。金属蛋白酶92 kDa明胶酶（MMP-9）与骨关节炎中细胞外基质的降解有关，但此前尚未研究BCP晶体诱导人成纤维细胞或成年猪软骨细胞中明胶酶的能力。本研究旨在探讨BCP晶体的促有丝分裂反应是否伴随着MMP-9的诱导和分泌这一假说。
方法
通过Northern印迹法和逆转录-聚合酶链反应（RT-PCR）检测MMP-9信使核糖核酸（mRNA）。通过蛋白质印迹法和条件培养基的酶谱分析鉴定明胶酶的分泌。
结果
BCP晶体在人成纤维细胞中引起MMP-9 mRNA积累和蛋白质分泌的浓度依赖性诱导，但在成年猪软骨细胞中未引起。
结论
BCP晶体诱导人成纤维细胞而非成年猪软骨细胞产生MMP-9。成纤维细胞MMP-9可能是与滑液BCP晶体相关的关节破坏的重要介质。
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